二抗是与一抗来源不同的，宿主体内制备的，能与一抗或一抗片段结合的抗体。
二抗可通过信号放大提高灵敏度，并为标记和检测提供了更大的灵活性，通常标记酶或荧光素等行使功能。
二抗可以耦联不同的标记物，如酶（辣根过氧化酶HRP和碱性磷酸酶AP或其衍生物APAAP，PAP），荧光基团（FITC, PE, Cy荧光素, BF荧光素）, 生物素和胶体金等。
标记二抗具有高特异性、高灵敏度等性质，被广泛用于免疫学实验中，选用哪种偶联物标记的二抗取决于具体的实验。
博奥森热销二抗，标记二抗：
山羊抗兔 IgG: HRP | FITC | BF488 | BF594 | BF555 | IRDye® 800CW | Biotin | Cy3
山羊抗小鼠 IgG: HRP | FITC | BF488 | BF594
兔抗鸡 IgG: FITC 兔抗山羊 IgG: HRP 兔抗猪 IgG: HRP
| 靶标物种 | 二抗名称 | 未标记 | 产品应用 | 标记类型 |
| Rabbit | Donkey anti-Rabbit IgG | bs-0295D | WB, IHC-P, IHC-F, ELISA |
AbBy Fluor® 350 AbBy Fluor® 405 AbBy Fluor® 488 AbBy Fluor® 555 AbBy Fluor® 594 AbBy Fluor® 647 AbBy Fluor® 680 AbBy Fluor® 750 HRP FITC Biotin RBITC PE PE-Cy5 PE-Cy5.5 PE-Cy7 APC APC-Cy5.5 APC-Cy7 Cy3 Cy5 Cy5.5 Cy7 PerCP PerCP-Cy5.5 IRDye 800CW AP/ALP Gold/Co-Au ATTO550 AE SA |
| Donkey anti-Rabbit IgG whole serum | bs-0295Ds | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Rabbit IgG | bs-0295G | - | ||
| Goat anti-Rabbit IgG whole serum | bs-0295Gs | WB, IHC-P, IHC-F, ELISA | ||
| AffiniPure F(ab')₂ Fragment Goat anti-Rabbit IgG (H+L) | bs-60295G | - | ||
| Mouse anti-Rabbit IgG (H + L) | bs-0295M | ELISA | ||
| Mouse anti-Rabbit IgG ascites | bs-0295Ms | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Rabbit IgG mAb | bsm-0295M | WB, IHC-P, IHC-F, ICC, IF, ELISA | ||
| Mouse anti-Rabbit IgM | bs-0369M | IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Avidin | bs-0312R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Avidin whole serum | bs-0312Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Biotin | bs-0311R | IHC-P, IHC-F, ELISA | ||
| Rabbit anti-BSA whole serum | bs-0292Rs | - | ||
| Rabbit anti-digoxin whole serum | bs-0356Rs | - | ||
| Mouse | Goat anti-Mouse IgG | bs-0296G | - | |
| Goat anti-Mouse IgG whole serum | bs-0296Gs | WB, IHC-P, IHC-F, ELISA | ||
| AffiniPure F(ab')₂ Fragment Goat anti-Mouse IgG (H+L) | bs-60296G | - | ||
| Goat anti-Mouse IgM | bs-0368G | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Mouse IgM whole serum | bs-0368Gs | WB, IHC-P, IHC-F, IF, ELISA | ||
| Rabbit anti-Mouse IgG | bs-0296R | - | ||
| Rabbit anti-Mouse IgG whole serum | bs-0296Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Mouse IgG-Fc | bs-0377R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Mouse IgG Fc whole serum | bs-0377Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Mouse Kappa light chain | bs-0330R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Mouse Kappa Chain whole serum | bs-0330Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Mouse IgM | bs-0368R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Mouse IgM whole serum | bs-0368Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rat anti-Mouse IgD | bsm-34233A | Flow-Cyt | ||
| Human | Human IgG-Fc | bsm-0365M | WB, IHC-P, IHC-F, ICC, IF, ELISA | |
| Donkey anti-Human IgG | bs-0297D | WB, IHC-P, IHC-F, ELISA | ||
| Donkey anti-Human IgG whole serum | bs-0297Ds | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Human IgG | bs-0297G | - | ||
| Goat anti-Human IgG whole serum | bs-0297Gs | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Human IgM | bs-0345G | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Human IgM whole serum | bs-0345Gs | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti Human IgE | bs-2226G | - | ||
| Goat anti-Human IgA whole serum | bs-0360Gs | - | ||
| Mouse anti-Human IgG | bs-0297M | ELISA | ||
| Mouse anti-Human IgG ascites | bs-0297Ms | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Human IgG mAb | bsm-41525M | ELISA | ||
| Mouse anti-Human IgG monoclonal antibody | bsm-0297M | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Human IgM | bs-0345M | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Human IgM | bsm-41524M | ELISA | ||
| Mouse anti-Human IgM ascites | bs-0345Ms | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgG | bs-0297R | - | ||
| Rabbit anti-Human IgG whole serum | bs-0297Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgG / Streptavidin | bsa-0297R | - | ||
| Rabbit anti-Human IgG F (ab')2 | bs-0364R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgG F(ab')₂ whole serum | bs-0364Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgG Fc | bs-0365R | WB, IHC-P, IHC-F, ICC, IF, ELISA | ||
| Rabbit anti-Human IgG Fc whole serum | bs-0365Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgG heavy chain constant region whole serum | bs-0411Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgM | bs-0345R | WB, IHC-P, IHC-F | ||
| Rabbit anti-Human IgM whole serum | bs-0345Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Human IgA | bs-0360R | ELISA | ||
| Rabbit anti-Human IgA whole serum | bs-0360Rs | - | ||
| Rat | Goat anti-Rat IgG | bs-0293G | - | |
| Goat anti-Rat IgG whole serum | bs-0293Gs | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Rat IgG | bs-0293M | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Rat IgG ascites | bs-0293Ms | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Rat IgG | bs-0293R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Rat IgG whole serum | bs-0293Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Rat IgM | bs-0346R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Rat IgM whole serum | bs-0346Rs | WB, IHC-P, IHC-F, ELISA | ||
| Goat | Donkey anti-Goat IgG | bs-0294D | WB, IHC-P, IHC-F, ELISA | |
| Donkey anti-Goat IgG whole serum | bs-0294Ds | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Goat IgG | bs-0294M | - | ||
| Mouse anti-Goat IgG ascites | bs-0294Ms | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti Goat IgG monoclonal antibody | bsm-0294M | WB, IHC-P, IHC-F, ICC, IF, ELISA | ||
| Mouse anti-Goat IgM ascites | bs-0370Ms | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Goat IgG | bs-0294R | - | ||
| Rabbit anti-Goat IgG whole serum | bs-0294Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Goat IgM | bs-0370R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Goat IgM whole serum | bs-0370Rs | WB, IHC-P, IHC-F, ELISA | ||
| Bovine | Goat anti-Bovine IgG | bs-0326G | WB, IHC-P, IHC-F, ELISA | |
| Goat anti-Bovine IgG whole serum | bs-0326Gs | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Bov IgG | bs-0326M | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Bovine IgG ascites | bs-0326Ms | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Bov IgG | bs-0326R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Bovine IgG whole serum | bs-0326Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Bovine IgM | bs-0327R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Bovine IgM whole serum | bs-0327Rs | WB, IHC-P, IHC-F, ELISA | ||
| Cat | Rabbit anti-Cat IgG H&L | bs-10749R | IHC-P, IHC-F, ELISA | |
| Chicken | Goat anti-Chicken IgG | bs-0310G | WB, IHC-P, IHC-F, ELISA | |
| Goat anti-Chicken IgG whole serum | bs-0310Gs | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Chicken IgY | bs-0432G | WB, ELISA | ||
| Mouse anti-Chicken IgG H&L | bs-0310M | - | ||
| Mouse anti-Chicken IgY | bs-0432M | WB, IHC-P, IHC-F, ICC, IF, ELISA | ||
| Rabbit anti-Chicken IgG | bs-0310R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Chicken IgG whole serum | bs-0310Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Chicken IgM | bs-0314R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Chicken IgM whole serum | bs-0314Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Chicken IgY | bs-0432R | WB, IHC-P, IHC-F, IF, ELISA | ||
| Dog | Rabbit anti-Dog IgG | bs-0303R | WB, IHC-P, IHC-F, ELISA | |
| Rabbit anti-Dog IgG whole serum | bs-0303Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Dog IgM | bs-0373R | IHC-P, IHC-F, IF, ELISA | ||
| Guinea pig | Donkey anti-Guinea pig IgG | bs-0358D | WB, IHC-P, IHC-F, ELISA | |
| Donkey anti-Gpig IgG whole serum | bs-0358Ds | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Guinea pig IgG | bs-0358G | WB, IHC-P, IHC-F, ELISA | ||
| Goat anti-Gpig IgG whole serum | bs-0358Gs | WB, IHC-P, IHC-F, ELISA | ||
| Mouse anti-Guinea pig IgG | bs-0358M | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Guinea pig IgG | bs-0358R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Guinea pig IgG whole serum | bs-0358Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Guinea pig IgM | bs-0375R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Guinea pig IgM whole serum | bs-0375Rs | WB, IHC-P, IHC-F, ELISA | ||
| Horse | Rabbit anti-Horse IgG | bs-0308R | WB, IHC-P, IHC-F, ELISA | |
| Rabbit anti-Horse IgG whole serum | bs-0308Rs | WB, IHC-P, IHC-F, ELISA | ||
| Mongolian Gerbil | Rabbit anti-MG IgG | bs-0403R | WB, IHC-P, IHC-F, ELISA | |
| Rabbit anti-MG IgG whole serum | bs-0403Rs | WB, IHC-P, IHC-F, ELISA | ||
| Mink | Rabbit anti-Mink IgG | bs-0777R | WB, IHC-P, IHC-F, ELISA | |
| Monkey | Rabbit anti-Monkey IgG | bs-0335R | WB, IHC-P, IHC-F, ELISA | |
| Rabbit anti-Monkey IgG whole serum | bs-0335Rs | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Monkey IgM | bs-0336R | WB, IHC-P, IHC-F, ELISA | ||
| Rabbit anti-Monkey IgM whole serum | bs-0336Rs | WB, IHC-P, IHC-F, ELISA | ||
| Pig | Rabbit anti-Pig IgG | bs-0309R | WB, IHC-P, IHC-F, ELISA | |
| Rabbit anti-Pig IgG whole serum | bs-0309Rs | WB, IHC-P, IHC-F, ELISA |
标记二抗高分文献展示
