| 产品编号 | bsm-60931R |
| 英文名称 | ATF3 Recombinant Rabbit mAb |
| 中文名称 | 活化转录因子3重组兔单抗 |
| 别 名 | Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription factor ATF-3; TF3_HUMAN; cAMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3 isoform 1. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 3G6 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATF |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]. SWISS: P18847 Gene ID: 467 转录调节因子（Transcriptin Regulators） 活化转录因子-3(属于activation transcription factor/cAMP responsive element binding protein家族)是真核细胞转录因子，同属于ATF/CREB家族。参与由cAMP或某些病毒蛋白质所诱导基因转录的调节。 转录激活因子3(activating transcription factor 3,ATF3)属含亮氨酸拉链结构的转录因子ATF/CREB家族成员，分子量20kD |
| 产品图片 |
RAW264.7 (M) cells were treated with or without LPS (1ug/ml) for 2 h, 25 μg total protein per lane of cell lysates (see on figure) probed with ATF3 monoclonal antibody, unconjugated (bsm-60931R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATF3 Monoclonal Antibody, Unconjugated (bsm-60931R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
