| 产品编号 | bsm-61107R |
| 英文名称 | Phospho-PP2A alpha (Ser307) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化蛋白磷酸酶2A重组兔单抗 |
| 别 名 | eplication protein C; PP2A-alpha; Replication protein C; RP-C; PPP2CA; MGC786. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | 35 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PP2A alpha around the phosphorylation site of S307: PD-pY-FL |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. SWISS: P67775 Gene ID: 5516 激酶和磷酸酶（Kinases and Phosphatases） PP-2A 蛋白质磷酸脂酶主要用于细胞信号传导，参与酵母细胞及两栖类卵母细胞有丝分裂的蛋白丝/苏氨酸磷酸酶。由65kDa和55kDa（亚基A和B）调节亚基及37kDa（亚基C）催化亚基组成。催化亚基在物种间高度保守。此酶的表达与细胞周期调节有关。此抗体与PP-2A subunit A,B 均有交叉反应。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PP2A alpha (Ser307) monoclonal antibody, unconjugated (bsm-61107R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
