| 产品编号 | bsm-61125R |
| 英文名称 | Adiponectin Recombinant Rabbit mAb |
| 中文名称 | 脂联素重组兔单抗 |
| 别 名 | ACDC; ACRP30; Adipocyte; Adiponectin; Adiponectin precursor; Adipoq; ADIPQTL1; ADPN; APM1; GBP28; Gelatin binding protein 28. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 10B12 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 26 kDa |
| 检测分子量 | 30 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Adiponectin: 57-100 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. SWISS: Q15848 Gene ID: 9370 脂联素又被称作Acrp30、apM1、AdipoQ、GBP28。 最初, 脂联素是在人体皮下脂肪组织、血浆和鼠科动物的脂肪细胞中被发现人体内的脂联素由244个氨基酸组成,分子量为27KD。 脂联素影响脂肪和糖类的代谢，还可以降低多种粘附分子(VCAM-1、ICAM-1、选择素-E) 在内皮细胞的表达。并影响着肿瘤坏死因子（TNF）分泌，近年来主要用于糖尿病的研究。 脂联素作为一种胰岛素超敏化激素(An Insulin–sensitizing Hormone),可以增加促进骨骼肌细胞的脂肪酸氧化和糖吸收,明 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Adiponectin monoclonal antibody, unconjugated (bsm-61125R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61125R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61125R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61125R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate tumors; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Adiponectin Monoclonal Antibody, Unconjugated (bsm-61125R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
