| 产品编号 | bsm-61145R |
| 英文名称 | p57 Kip2/Cdkn1c Recombinant Rabbit mAb |
| 中文名称 | 周期蛋白依赖激酶抑制因子1C重组兔单抗 |
| 别 名 | BWCR; BWS; KIP2; WBS; p57; p57 Kip2; WBS; CDKN1C; Cyclin dependent kinase inhibitor 1C. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32 kDa |
| 检测分子量 | 57 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human p57 Kip2: 45-72 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. SWISS: P49918 Gene ID: 1028 p57 Kip-2调控周期蛋白依赖蛋白激酶、G1 期, 是细胞周期蛋白依赖性激酶(CDK)的抑制蛋白。它通过调控细胞周期进程，参与肿瘤细胞的增殖、分化与凋亡。在多种肿瘤中均发现p57,kip2表达异常，在某些肿瘤中是一种独立的预后因素，与肿瘤的发生、发展及预后有着密切关系。 |
| 产品图片 |
Western blot analysis of HeLa cells Kip2 Antibody. Using p57 Kip2/Cdkn1c (bsm-61145R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with p57 Kip2/Cdkn1c Monoclonal Antibody, Unconjugated(bsm-61145R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
