产品编号 | bsm-61181R |
英文名称 | beta 4B/2A/2B Tubulin Recombinant Rabbit mAb |
中文名称 | beta 4B/2A/2B微管蛋白重组兔单抗 |
别 名 | class II beta tubulin isotype; beta2; Tubulin beta 2 chain; TUBB; TUBB2. |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | |
产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 50 kDa |
检测分子量 | 50 |
性 状 | Liquid |
免 疫 原 | A synthesized peptide derived from human beta 4B Tubulin: 400-445/445 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. SWISS: P68371,Q13885,Q9BVA1 Gene ID: 10383 |
产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with beta 4B/2A/2B Tubulin monoclonal antibody, unconjugated (bsm-61181R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |