| 产品编号 | bsm-61219R |
| 英文名称 | Midkine Recombinant Rabbit mAb |
| 中文名称 | 中期因子/肝素结合生长因子重组兔单抗 |
| 别 名 | MDK; FLJ27379; MK1; NEGF2; Midkine; NEGF2; ARAP. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 16 kDa |
| 检测分子量 | 16 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Midkine: 120-143 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Midkine, or MK, is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis. MK expression is tightly regulated during embryonic development by steroid receptors of the retinoic acid superfamily. The mature human MK protein is 118 amino acids in length and contains five intrachain disulfide bonds. MK is a non-glycosylated protein that shows greater than 87% identity between human and mouse. SWISS: P21741 Gene ID: 4192 中期因子(midkine, MK)具有多种生物学功能: 可促进神经轴突 的生长和各种胚胎神经元的存活,做为一种有丝分裂原,与肿瘤的发生密切相关，MK还有较强的 促血管生成特性, 经研究证实, MK可促进内皮细胞增殖并 提高血管的密度，在多种肿瘤组织和肿 瘤患者的血清中的水平都明显升高。MK和多向促激素(p leiotro2 phin, PTN)同属于一类新的结合肝素的生长因子家 族。 |
| 产品图片 |
Western blot analysis of Midkine Recombinant protein. Using Midkine (bsm-61219R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
