| 产品编号 | bsm-61287R |
| 英文名称 | ILK Recombinant Rabbit mAb |
| 中文名称 | 整合素连接激酶ILK重组兔单抗 |
| 别 名 | ILK; DKFZp686F1765; P59; ILK1; Integrin linked protein kinas. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 10G2 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | 51 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ILK: 401-452/452 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. SWISS: Q13418 Gene ID: 3611 ILK 是一种新发现的Ser/Thr蛋白激酶。ILK能够通过与整合素β1亚单位的结合介导细胞与胞外基质的连接，以依赖于PI3K的方式激活，并通过磷酸化下游底物PKB/AKT,GSK3等胞外信号的一项下游传递，对细胞的生长，分化，迁移等进行调控。由于ILK在胞内外信号传导中起着重要的作用。并且抑制ILK的活性能够导致细胞周期的停滞和细胞程序性死亡的启动，使其成为肿瘤治疗和肿瘤药物的理想靶位点。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with ILK monoclonal antibody, unconjugated (bsm-61287R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
