| 产品编号 | bsm-61354R |
| 英文名称 | Phospho-CBL (Tyr774) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化原癌蛋白CBL2重组兔单抗 |
| 别 名 | Casitas B lineage lymphoma proto oncogene; CBL2; E3 ubiquitin protein ligase CBL; Oncogene CBL2; Proto oncogene c CBL; RING finger protein 55; RNF55; Signal transduction protein CBL. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 100 kDa |
| 检测分子量 | 120 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human CBL around the phosphorylation site of Y774: DG-pY-DV |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. SWISS: P22681 C CBL是泛素一蛋白酶体(ubiquitin proteasome)通路中的一个新的RINGFinger型泛素连接酶(ubiquitinligase ,E3).C CBL可以介导受体酪氨酸激酶和非受体酪氨酸受体激酶的降解. |
| 产品图片 |
Western blot analysis of Jurkat cell treated with Pervanadate lysate. Using Phospho-CBL (Tyr774) (bsm-61354R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
