| 产品编号 | bsm-61358R |
| 英文名称 | MGMT Recombinant Rabbit mAb |
| 中文名称 | O6甲基鸟嘌呤DNA甲基转移酶重组兔单抗 |
| 别 名 | MGMT |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22 kDa |
| 检测分子量 | 22 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MGMT: 1-45/207 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. SWISS: P16455 Gene ID: 4255 O-6-methylguanine-DNA methyltransferase(MGMT)O-6-甲基鸟嘌呤-DNA-甲基转移酶:是广泛存在于细菌到哺乳动物机体中的一种独特DNA修复酶，MGMT是一种DNA修复酶，是突变和癌变的抑制因子。它对烷化剂导致的DNA损伤有修复作用。 MGMT是突变和癌基因的抑制因子。研究表明MGMT水平与恶性肿瘤的发生、进展、浸润、预后及治疗有密切关系。细胞中MGMT水平与其所能耐受的DNA损伤密切相关。一般来讲，低水平表达MGMT的肿瘤可以应用烷化剂药物治疗；反之则为耐药。在 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MGMT monoclonal antibody, unconjugated (bsm-61358R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MGMT Monoclonal Antibody, Unconjugated(bsm-61358R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MGMT Monoclonal Antibody, Unconjugated(bsm-61358R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
