| 产品编号 | bsm-61405R |
| 英文名称 | Parvalbumin Recombinant Rabbit mAb |
| 中文名称 | 细小清蛋白重组兔单抗 |
| 别 名 | pvalb1; ATH; OCM2; D22S749; PV; Parv; Pva; PALB1; PRVA_HUMAN; PVALB; Alpha-parvalbumin (Alpha-PV); PRVA_MOUSE; PRVA_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 11H20 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12 kDa |
| 检测分子量 | 12 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Parvalbumin alpha: 1-110/110 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. SWISS: P20472 Gene ID: 5816 (Parvalbumin, PV)细小白蛋白属于钙结合蛋白家族,是一种细胞内可溶的小结合蛋白，过去曾认为，仅出现在哺乳动物某些肌纤维内,它与Ca2+有高度亲合力,与钙调蛋白竞争自由Ca2+,故可阻止肌浆内的Ca2+增高，可抗衡细胞内钙过量的发生。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Parvalbumin monoclonal antibody, unconjugated (bsm-61405R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
