| 产品编号 | bsm-61410R |
| 英文名称 | NUMB Recombinant Rabbit mAb |
| 中文名称 | 膜相关蛋白Numb重组兔单抗 |
| 别 名 | c14_5527; h Numb; Numb; Numb homolog; S171. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71 kDa |
| 检测分子量 | 71 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NUMB: 1-47/651 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch. The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates. The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling. SWISS: P49757 Gene ID: 8650 膜相关蛋白Numb是细胞命运决定因子：在细胞有丝分裂期，Numb选择性地分布于细胞的一侧,在胞质分裂后则被分配于一个子细胞，Numb通过抑制跨膜受体Notch而发挥作用, Numb信号途径在前体细胞的神经元调控方面起着重要作用. |
| 产品图片 |
Western blot analysis of Ramos cell lysate. Using NUMB (bsm-61410R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
