| 产品编号 | bsm-61454R |
| 英文名称 | Tyrosinase Recombinant Rabbit mAb |
| 中文名称 | 酪氨酸酶重组兔单抗 |
| 别 名 | ATN; CMM8; LB24 AB; SHEP3; SK29 AB; Tumor rejection antigen AB; TYR. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 20C5 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 检测分子量 | 70-80 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Tyrosinase: 1-300/529 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. SWISS: P14679 Gene ID: 7299 酪氨酸酶( Tyrosinase) 又称酚氧化酶、多酚氧化酶、儿茶酚氧化酶,是结构复杂的多亚基的含铜氧化还原酶,广泛存在于微生物、动植物及人体中。 酪氨酸酶具有独特的双重催化功能,是生物体内黑色素合成的关键酶,与人的衰老,昆虫的伤口愈合与发育,果蔬的褐变有密切关系。多年来,酪氨酸酶一直受到国内外的关注,其研究涉及生物、医学、农学、化学、药学等多个学科和领域.酪氨酸酶作为黑色素合成的关键酶,其异常过量表达可导致人体的色素沉着性疾病。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Tyrosinase monoclonal antibody, unconjugated (bsm-61454R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
