| 产品编号 | bsm-61456R |
| 英文名称 | Hepcidin-25 Recombinant Rabbit mAb |
| 中文名称 | 铁调节蛋白25重组兔单抗 |
| 别 名 | Hepcidin; Liver-expressed antimicrobial peptide 1; LEAP-1; Putative liver tumor regressor; PLTR; Hepcidin-25; Hepc25; Hepcidin-20; Hepc20; HAMP; HEPC; LEAP1. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 9 kDa |
| 检测分子量 | 25 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Hepcidin: 1-84/84 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Hepcidin, also known as HAMP, HEPC, LEAP1 or HFE2B, is an 84 amino acid secreted protein that regulates iron-related signaling events. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. SWISS: P81172 Gene ID: 57817 Hepcidin是一种由肝脏细胞产生的具有广谱杀菌抑菌作用的抗菌肽，属于防御性蛋白家族。Hepcidin在肝脏合成时，首先产生一种早期多肽，由84个氨基酸残基组成。在其Gly24和Ser25之间存在一个酶切位点，当酶切去除其24个氨基酸残基的信号肽后，形成由60个氨基酸残基组成的前体肽(pro-Hepcidin)，并通过转运进入血液循环，最后由前肽转化酶(propeptide convertase)在N端的不同位点剪接生成3种不同的成熟小肽，分别由20、22、25个氨基酸组成，其中以25个氨基酸为其主要形 |
| 产品图片 |
Western blot analysis of Human Hepcidin Full-length Recombinant Protein (GFP Tagged). Using Hepcidin-25 (bsm-61456R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
