| 产品编号 | bsm-61460R |
| 英文名称 | Ku70 / XRCC6 Recombinant Rabbit mAb |
| 中文名称 | DNA修复酶Ku70重组兔单抗 |
| 别 名 | CTC75; CTCBF; G22P1; KU70; ML8; TLAA; 70kDa; XRCC6_HUMAN; XRCC6; 5'-deoxyribose-5-phosphate lyase Ku70 (5'-dRP lyase Ku70); 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit (CTC75 | CTCBF); DNA repair protein XRCC6; Lupus Ku autoantigen protein p70 (Ku70); Thyroid-lupus autoantigen (TLAA); X-ray repair complementing defective repair in Chinese hamster cells 6; 3.6.4.-; 4.2.99.-; XRCC6_MOUSE; 5'-deoxyribose-5-phosphate lyase Ku70 (5'-dRP/AP lyase Ku70); Ku autoantigen protein p70 homolog (Ku70); |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 1C4 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70 kDa |
| 检测分子量 | 70 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Ku70: 525-568 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. SWISS: P12956 Gene ID: 2547 Ku-70是一种DNA修复蛋白，当细胞在受到辐射损伤而发生DNA双链断裂时，Ku70可迅速将其修复，从而提高细胞存活率. Ku是一种多功能的蛋白，在许多重要的细胞生命过程中起着直接或间接的作用，如DNA双链断裂的修复，免疫球蛋白和T细胞受体V（D）J重排，免疫球蛋白构型转换，DNA复制，DNA转录的调节，同时在细胞周期的G2和M时相中起着特殊的作用. |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Ku70 / XRCC6 monoclonal antibody, unconjugated (bsm-61460R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 / XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
