| 产品编号 | bsm-61465R |
| 英文名称 | XRCC1 Recombinant Rabbit mAb |
| 中文名称 | X射线修复交叉互补蛋白重组兔单抗 |
| 别 名 | DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; XRCC 1. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 5C16 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | 90 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human XRCC1: 1-50/633 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. SWISS: P18887 Gene ID: 7515 XRCC-1是一种重要的DNA修复基因,在一系列内外氧化剂(包括烟草成分)导致的DNA单链断裂和碱基损伤修复中起到重要作用,是目前肿瘤研究的重要的蛋白。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with XRCC1 monoclonal antibody, unconjugated (bsm-61465R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (XRCC1) monoclonal Antibody, unconjugated (bsm-61465R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
