| 产品编号 | bsm-61576R |
| 英文名称 | RASSF1A Recombinant Rabbit mAb |
| 中文名称 | Ras相关区域家族1A重组兔单抗 |
| 别 名 | NORE2A |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | 39 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human RASSF1: 300-344/344 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. SWISS: Q9NS23 Gene ID: 11186 RASSF1A是一个肿瘤抑制基因，RASSF1A蛋白的表达能显著抑制肿瘤细胞的生长和转移， 作为一种候选肿瘤抑制基因参与肿瘤的发生发展 ,该基因的失活与其启动子区甲基化有关。 该蛋白在恶性肿瘤的发生发展中发挥重要作用。 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using RASSF1A (bsm-61576R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
