| 产品编号 | bsm-61594R |
| 英文名称 | BCHE Recombinant Rabbit mAb |
| 中文名称 | 丁酰胆碱酯酶重组兔单抗 |
| 别 名 | BCHED; CHE1; CHE2; E1; CHLE_HUMAN; BCHE; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase; 3.1.1.8; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 4B3 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 68 kDa |
| 检测分子量 | 90 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Cholinesterase: 570-602 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. SWISS: P06276 Gene ID: 590 血清丁酰胆碱酯酶是由四个亚基组成的糖蛋白，分子量28万～35万。作为一类糖蛋白，主要存在于脑、肝、血及胆碱能神经末稍的突触间隙中，主要由肝脏合成，并存在于血清中，生理作用不明，称为假性胆碱酯酶或丁酰胆碱酯酶[1]Lockridge等采用Edman降解法测定了人血清BChE完整的氨基酸序列，共含有574个氨基酸残基。BChE氨基酸序列与其他酯酶家族蛋白有很高的同源性。以电鳐AChE的三维结构为模板对人BChE的三维结构进行计算机模拟结果表明，BChE活性中心，囊袋的底部为Ser198，其两侧相距一定距离分别 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with BCHE monoclonal antibody, unconjugated (bsm-61594R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
