| 产品编号 | bsm-61609R |
| 英文名称 | HSD11B1 Recombinant Rabbit mAb |
| 中文名称 | 羟基类固醇脱氢酶11β1重组兔单抗 |
| 别 名 | 11-DH; 11-beta-HSD1; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1; LRRGT00065; DHI1_MESAU; HSD11B1; 7-alpha-hydroxycholesterol dehydrogenase (7-alpha-HCD); 7-oxosteroid reductase; Corticosteroid 11-beta-dehydrogenase isozyme 1; 1.1.1.146; DHI1_HUMAN; Short chain dehydrogenase/reductase family 26C member 1; DHI1_MOUSE; 11-beta-hydroxysteroid dehydrogenase/microsomal carbonyl reductase (11-beta-HSD1A/MCR); 11beta-HSD1A; liver-type 11-beta-HSD; DHI1_RABIT; DHI1_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 1D11 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32 kDa |
| 检测分子量 | 32 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human HSD11B1: 1-57/292 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. SWISS: P28845 Gene ID: 3290 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with HSD11B1 monoclonal antibody, unconjugated (bsm-61609R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSD11B1 Monoclonal Antibody, Unconjugated (bsm-61609R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSD11B1 Monoclonal Antibody, Unconjugated (bsm-61609R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSD11B1 Monoclonal Antibody, Unconjugated (bsm-61609R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
