| 产品编号 | bsm-61651R |
| 英文名称 | TRPM8 Recombinant Rabbit mAb |
| 中文名称 | “冷”蛋白TRPM8重组兔单抗 |
| 别 名 | LTrpC6; MGC2849; Transient receptor potential p8; Trp p8; Trpm8; TRPP8. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 128 kDa |
| 检测分子量 | 128 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TRPM8: 900-1000 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. SWISS: Q7Z2W7 Gene ID: 79054 TRPM8与感应热和辣的蛋白质属于同一家族，它存在于特定神经细胞的细胞膜上，成为响应外界信号的开关通道。科学家研究发现，当温度低于27摄氏度或者醇存在的条件下，TRPM8通道都会打开，使钙离子等带正电的粒子进入细胞。 TRPM8的蛋白质在产生冷知觉的过程中起着主导作用，并且控制着低温对机体疼痛的缓解效果, TRPM8故称是“冷”蛋白。 |
| 产品图片 |
Western blot analysis of A549 cell lysate. Using TRPM8 (bsm-61651R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
