产品编号 | bsm-61699R |
英文名称 | CRP Recombinant Rabbit mAb |
中文名称 | C-反应蛋白重组兔单抗 |
别 名 | CRP; Pentraxin 1; PTX1; C reactive protein pentraxin related. |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | 20F17 |
产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 25 kDa |
检测分子量 | 25 |
性 状 | Liquid |
免 疫 原 | A synthesized peptide derived from human C reactive protein: 201-224/224 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. SWISS: P02741 Gene ID: 1401 C反应蛋白 CRP(C-reactive protin)是与C性肺炎球菌的多糖起反应的一种蛋白,在某些细菌的感染过程中出现在血浆中。 CRP是在一些病理性情况下出现于病人血清中的一种糖蛋白,特别是在伴有肯炎症和组织坏死疾病的急性期。血清中出现的这种异常蛋白能和肺炎C-多糖体发生沉淀反应,当疾病好转或缓解时,CRP减低或消失。 临床意义: (1)各种急性化脓性炎症、菌血症、组织坏死(心肌梗塞、手术、严重创伤、烧伤等)、恶性肿瘤、结缔组织病、肾移殖后急性排异反应、急性风湿热、风湿性关节炎、类风湿性关节炎、心 |
产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CRP monoclonal antibody, unconjugated (bsm-61699R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CRP Monoclonal Antibody, Unconjugated (bsm-61699R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CRP Monoclonal Antibody, Unconjugated (bsm-61699R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |