| 产品编号 | bsm-61719R |
| 英文名称 | Heparanase Recombinant Rabbit mAb |
| 中文名称 | 乙酰肝素酶重组兔单抗 |
| 别 名 | HEP; Heparanase; Heparanase1; Hpa 1; HPA; Hpa1 HPR1; HPSE1; HSE1. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61 kDa |
| 检测分子量 | 70 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Heparanase: 1-60/543 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. SWISS: Q9Y251 Gene ID: 10855 乙酰肝素酶(heparanase)在血管形成、炎性反应、组织生长发育及肿瘤转移的过程中起着重要作用。在正常情况下,乙酰肝素酶的分泌和活性必须受到严格的调控,以免其表达对细胞外基质错误切割造成组织损伤而发生各种病理改变。 |
| 产品图片 |
Western blot analysis of K562 cell lysate. Using Heparanase (bsm-61719R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
