| 产品编号 | bsm-61756R |
| 英文名称 | PU.1/Spi1 Recombinant Rabbit mAb |
| 中文名称 | 造血转录因子PU.1重组兔单抗 |
| 别 名 | oncogene spi1; PU.1; SFPI1; SPI 1; SPI A; Spi1. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 16B14 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | 42 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SPI1: 1-50/270 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions. SWISS: P17947 Gene ID: 6688 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with PU.1/Spi1 monoclonal antibody, unconjugated (bsm-61756R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
