| 产品编号 | bsm-61772R |
| 英文名称 | LIMK1 Recombinant Rabbit mAb |
| 中文名称 | LIM结构域激酶1重组兔单抗 |
| 别 名 | LIM kinase; LIMK 1; LIMK. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 7B6 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 73 kDa |
| 检测分子量 | 72 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human LIMK 1: 1-57/647 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. SWISS: P53667 Gene ID: 3984 LIM激酶1(LIMkinase1,LIMK-1)是单丝氨酸蛋白激酶,LIMK-1的活化受多种机制调控,成为联系细胞外刺激与细胞骨架稳定性的枢纽,在多种基本的生物学过程中起重要作用。LIMK-1包含LIM和PDZ蛋白和蛋白相互作用区、P/S结构域、激酶结构域,在神经元中呈高表达。它的主要作用是使肌动蛋白解聚因子cofilin磷酸化和失活,从而导致肌动蛋白细胞骨架的改变。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with LIMK1 monoclonal antibody, unconjugated (bsm-61772R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
