| 产品编号 | bsm-61825R |
| 英文名称 | EIF2AK4/GCN2 Recombinant Rabbit mAb |
| 中文名称 | 蛋白激酶GCN2蛋白重组兔单抗 |
| 别 名 | GCN2; PVOD2; 2610011M03; MGCN2; E2AK4_HUMAN; EIF2AK4; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; 2.7.11.1; KIAA1338; E2AK4_MOUSE; GCN2-like protein (mGCN2); eukaryotic translation initiation factor 2 alpha kinase 4; eIF-2-alpha kinase |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 11H4 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 187kDa |
| 检测分子量 | 187 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human GCN2: 1-59/1649 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability.Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. SWISS: Q9P2K8 Gene ID: 440275 GCN2蛋白的作用；可能是将短期记忆变成长期记忆的一个主控调节因子， GCN2蛋白在长期记忆形成中功能的发现将可能帮助研究人员开发出提高记忆力丧失的病人提高记忆力。 |
| 产品图片 |
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (EIF2AK4/GCN2) monoclonal Antibody, unconjugated (bsm-61825R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
