产品编号 | bsm-61831R |
英文名称 | IL-23 Recombinant Rabbit mAb |
中文名称 | 白介素-23重组兔单抗 |
别 名 | IL 23A; IL 23p19; IL12B; IL23; Il23a; IL23P19; P19; SGRF. |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | 15E15 |
产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 21 kDa |
检测分子量 | 21 |
性 状 | Liquid |
免 疫 原 | A synthesized peptide derived from human IL 23A: 118-189 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity.Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4. SWISS: Q9NPF7 Gene ID: 51561 IL23(Interleukin-23)作为一种前炎症细胞因子,具有影响机体免疫反应和抗感染作用,白介素23能连接肿瘤关联的炎症和肿瘤免疫监督缺乏关系的细胞因子。存在于很多人类肿瘤中,能通过促进血管形成来帮助肿瘤生长。 |
产品图片 |
293T (H) cells were treated with or without LPS and IFN (40ng/mL) for 24 h, BFA (300ng/mL) for 24 h, 25 μg total protein per lane of cell lysates (see on figure) probed with IL-23 polyclonal antibody, unconjugated (bsm-61831R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |