| 产品编号 | bsm-61924R |
| 英文名称 | IQGAP1 Recombinant Rabbit mAb |
| 中文名称 | 支架蛋白重组兔单抗 |
| 别 名 | Iqgap1; p195; SAR1. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 189 kDa |
| 检测分子量 | 180 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IQGAP1: 1-58/1657 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. SWISS: P46940 Gene ID: 8826 支架蛋白IQGAP1与许多重要的细胞活动紧密相关，如黏附和迁移。研究发现在损伤修复、组织发展、肿瘤转移等过程中，IQGAP1的表达和功能有明显改变。同时作为Rh.GTPase信号的主要效应因子，IQGAP1能传递细胞外信号至黏附素复合体、细胞骨架以及细胞核。通过上述信号通路，IQ-GAP1协助细胞完成一系列重要生理病理活动。 |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using IQGAP1 (bsm-61924R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
