产品编号 | bsm-62143R |
英文名称 | PAR-2 Recombinant Rabbit mAb |
中文名称 | 蛋白酶激活受体-2重组兔单抗 |
别 名 | F2RL1; GPR11; PAR 2. |
抗体来源 | Rabbit |
克隆类型 | |
克 隆 号 | 16C12 |
产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 44 kDa |
检测分子量 | 55 |
性 状 | Liquid |
免 疫 原 | A synthesized peptide derived from human PAR2: 330-397/397 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Receptor for trypsin and trypsin-like enzymes coupled to G proteins that stimulate phosphoinositide hydrolysis. May have a role in the regulation of vascular tone. SWISS: P55085 Gene ID: 2150 PAR2也是细胞表面受体,通过G蛋白影响细胞功能,PAR蛋白全称为蛋白酶活化受体(Protease-activated receptors),是独立家族的G蛋白偶联受体,经研究认为PAR2与炎症,实体肿瘤等密切相关。 |
产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with PAR-2 monoclonal antibody, unconjugated (bsm-62143R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(bsm-62143R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(bsm-62143R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(bsm-62143R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(bsm-62143R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(bsm-62143R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAR-2 Monoclonal Antibody, Unconjugated(bsm-62143R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |