| 产品编号 | bsm-62165R |
| 英文名称 | Pokemon/ZBTB7A Recombinant Rabbit mAb |
| 中文名称 | 扑克蒙蛋白重组兔单抗 |
| 别 名 | FBI1; LRF; Pokemon; TIP21; ZBTB7; ZBTB7A; ZNF857A. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 2B1 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 61 kDa |
| 检测分子量 | 70 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ZBTB7A: 520-584 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. SWISS: O95365 Gene ID: 51341 Pokemon(7APOK Erythroid Myeloid Ontogenic factor)能够控制将正常细胞变成癌细胞所需的途径,Pokemon蛋白是转录因子蛋白家族的一员，并且在人类癌症中发生了突变。另外Pokemon蛋白作为转录因子参与一些细胞基因转录的调节,并在细胞分化过程中发挥着关键、多效性的功能。近来发现,Poke-mon在致癌转化过程中发挥着至关重要的功能,并与肿瘤的发生密切相关。 这种蛋白很可能在实质肿瘤中处于重要地位,还有学者认为：Pokemon在一定类型的B细胞和T细胞淋巴瘤中水 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Pokemon/ZBTB7A monoclonal antibody, unconjugated (bsm-62165R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation Pokemon/ZBTB7A Monoclonal Antibody, Unconjugated(bsm-62165R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
