| 产品编号 | bsm-62224R |
| 英文名称 | MYF5 Recombinant Rabbit mAb |
| 中文名称 | 生肌决定因子Myf5重组兔单抗 |
| 别 名 | Myf5; Class C basic helix loop helix protein 2. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 4G8 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | 37 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MYF5: 201-255/255 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. SWISS: P13349 Gene ID: 4617 yf5是MyoD基因家族的一员，该家族的共同结构特点是具有1个保守的中央蛋白基序(motif)，大小为80个氨基酸，称为碱性螺旋-环-螺旋(bHLH)，bHLH高度保守，其中Basic结构是HLH螺旋结构的延伸，也是这一家族蛋白与DNA相互作用的区域，而HLH螺旋结构则是与其他因子相互作用的位点，即调控的重要区域。 生肌决定因子(MyoD)基因家族控制着肌细胞的增殖和分化，与肌纤维的数量、大小有着密切的关系，因而对肉质和风味有非常重要的作用，MyoD基因家族可编码4种不同的转录因子，分别为MyoD(Myf |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MYF5 monoclonal antibody, unconjugated (bsm-62224R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
