| 产品编号 | bsm-62258R |
| 英文名称 | Phospho-GSK3 A/B (Tyr279/Tyr216) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化糖原合酶激酶3α/β重组兔单抗 |
| 别 名 | Glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK3A. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51,47 kDa |
| 检测分子量 | 51,46 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human GSK3 alpha around the phosphorylation site of Y279: VS-pY-IS |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
GSK3A a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. SWISS: P49840 Gene ID: 2931 组成性活性蛋白激酶，通过磷酸化和失活糖原合成酶（GYS1或GYS2）、CTNNB1/β连环蛋白、APC和AXIN1，在激素控制葡萄糖稳态、Wnt信号传导和转录因子及微管的调节中作为负调节因子。它的大多数底物需要启动磷酸化。通过磷酸化和抑制GYS1活性从而促进糖原合成的胰岛素调节。调节肝脏的糖原代谢，而不是肌肉。也可能通过调节转录因子的激活来介导胰岛素抵抗的发展。在Wnt信号中，调节核CTNNB1/β连环蛋白的水平和转录活性。促进淀粉样前体蛋白（APP）的加工和阿尔茨海默病APP源性淀粉样斑块的形成。可能参 |
| 产品图片 |
Western blot analysis of 293 cell lysate treated with AP. Using Phospho-GSK3 A/B (Tyr279/Tyr216) (bsm-62258R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
