| 产品编号 | bsm-62291R |
| 英文名称 | DP1 Recombinant Rabbit mAb |
| 中文名称 | 转录因子DP1重组兔单抗 |
| 别 名 | DILC; DP1; DRTF1; Dp-1; TFDP1_HUMAN; TFDP1; DRTF1-polypeptide 1 (DRTF1); E2F dimerization partner 1; Q5JSB4_HUMAN; TFDP1_MOUSE; DRTF1-polypeptide 1; Q3V3X3_MOUSE; Q9CYZ7_MOUSE; Q9D297_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 7C3 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | 50 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human DP1: 351-410/410 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication SWISS: Q5JSB4 Gene ID: 7027 |
| 产品图片 |
Western blot analysis of Raji cell lysate. Using DP1 (bsm-62291R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with DP1 monoclonal antibody, unconjugated (bsm-62291R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-DP1 antibody (bsm-62291R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
