| 产品编号 | bsm-62349R |
| 英文名称 | LCAT Recombinant Rabbit mAb |
| 中文名称 | 卵磷酯胆固醇酰基转移酶重组兔单抗 |
| 别 名 | LCAT |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 11E10 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50 kDa |
| 检测分子量 | 55 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human LCAT: 21-50 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). SWISS: P04180 Gene ID: 3931 LCAT是参与体内脂质代谢的主要成份之一，是一种在高密度脂蛋白(HDL)代谢和动脉粥样硬化(AS)发展中的关键酶。LCAT需要经载脂蛋白ApoAI、载脂蛋白D(ApoD)作为辅助因子并经ApoE活化来发挥作用。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with LCAT monoclonal antibody, unconjugated (bsm-62349R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
