| 产品编号 | bsm-62384R |
| 英文名称 | MICA Recombinant Rabbit mAb |
| 中文名称 | MHC I类链相关蛋白A/组织相容性复合物MHCIa重组兔单抗 |
| 别 名 | PERB11.1; MICA. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 18A14 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | 42-70 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MIC A: 80-134 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. SWISS: Q29983 Gene ID: 4276 MICA蛋白是一种细胞应激分子，在正常组织中表达量少，仅在肠道上皮组织表达量稍高，但是在多种肿瘤细胞，尤其是上皮源性的肿瘤如肺癌、乳腺癌、肾癌、卵巢癌、结肠癌等细胞表面高表达，并在细胞发生癌变的早期阶段出现在细胞表面。 该分子与相应受体NKG2D结合后可激活NK细胞、Vd1 gd T细胞、CD8+ T细胞的细胞毒作用，从而产生杀伤肿瘤细胞的生物效应。因此，抗MICA特异性抗体在肿瘤早期诊断、治疗中可能具有更高的敏感性和疗效，从而可实现肿瘤的“早诊断、早治疗”。并且还能对其它治疗方案的疗效进行早期评估。 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MICA monoclonal antibody, unconjugated (bsm-62384R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
