| 产品编号 | bsm-62434R |
| 英文名称 | TERT Recombinant Rabbit mAb |
| 中文名称 | 端粒酶反转录酶重组兔单抗 |
| 别 名 | TCS1; HEST2; EST2; TRT; Telomerase reverse transcriptase; Telomerase-associated protein 2; Telomerase catalytic subunit. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 127 kDa |
| 检测分子量 | 127 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TERT: 1000-1132 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. SWISS: O14746 Gene ID: 7015 端粒酶逆转录酶hTERT是构成端粒酶的组分之一，是端粒酶活性的必需和限速成分，水平决定细胞端粒酶的活性。抑制hTERT可降低端粒酶的活性，从而抑制瘤细胞生长。目前对hTERT的研究已成为端粒酶研究的热点问题，已发现TERT蛋白表达在肿瘤诊断中有重要意义，并制备了hTERT抗体及应用核酶技术等来抑制hTERT蛋白的表达，抑制端粒酶活性，从而抑制肿瘤的生长。 端粒反转录酶又称端粒酶催化亚单位 （hTRT；Telomerase catalytic subunit；HEST2；Telomerase-associa |
| 产品图片 |
Western blot analysis of Hela cells lysate. Using TERT (bsm-62434R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
