| 产品编号 | bsm-62566R |
| 英文名称 | Phospho-ACACA (Ser80) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化乙酰辅酶A羧化酶重组兔单抗 |
| 别 名 | ACAC; ACACA; ACACB; ACC alpha; ACC; ACC beta; ACC1; ACC2; ACCA; ACCB; Acetyl CoA carboxylase 1; Acetyl CoA carboxylase 2; COA1; COA2. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 266 kDa |
| 检测分子量 | 265 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Acetyl CoA carboxylase 1 around the phosphorylation site of S79: SM-pS-GL |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis.This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. SWISS: Q13085 Gene ID: 32 |
| 产品图片 |
Western blot analysis of (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate. Using Phospho-ACACA (Ser80) (bsm-62566R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
