| 产品编号 | bsm-62671R |
| 英文名称 | TRIM24 Recombinant Rabbit mAb |
| 中文名称 | 转录中介因子Tif1α重组兔单抗 |
| 别 名 | hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA; Trim24. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 117 kDa |
| 检测分子量 | 130 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TRIM24: 405-455 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). SWISS: O15164 Gene ID: 8805 与许多核受体和辅活化子相互作用并调节靶基因转录的转录辅活化子。依赖于组蛋白H3修饰与染色质相互作用，对组蛋白H3具有最高亲和力，组蛋白H3在“Lys-4”（H3K4me0）处未修饰并且在“Lys-23”（H3K23ac）处乙酰化。具有E3蛋白泛素连接酶活性，促进p53/TP53泛素化和蛋白酶体降解。在细胞增殖和凋亡的调节中发挥作用，至少部分通过其对p53/TP53水平的影响。通过AR、GCR/NR3C1、甲状腺激素受体（TR）和ESR1上调配体依赖性转录激活。通过维甲酸（RA）受体调节转录激活，包括RAR |
| 产品图片 |
Western blot analysis of HeLa cell lysate. Using TRIM24 (bsm-62671R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
