| 产品编号 | bsm-62758R |
| 英文名称 | Phospho-c Jun/JunD (Ser73/Ser100) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化原癌基因c-Jun/JunD重组兔单抗 |
| 别 名 | JunD; c-Jun. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 15D9 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36,35 kDa |
| 检测分子量 | 38,42,45 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human c Jun around the phosphorylation site of S73: LA-pS-PE |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity. SWISS: P05412 Gene ID: 3725 转录调节因子（Transcriptin Regulators） C-jun(Oncoprotein C-jun：active protein 1)基因与鸟类肉瘤病毒17的转化基因具有同源性，是早期应答基因家族成员之一。主要用于各种恶性肿瘤的研究。C-jun又称应激活化蛋白激酶. |
| 产品图片 |
Western blot analysis of RAW264.7 cell lysate. Using Phospho-c-Jun (Thr91+Thr93) (bsm-62758R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-c-Jun (Thr91+Thr93) Monoclonal Antibody, Unconjugated(bsm-62758R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
