| 产品编号 | bsm-62840R |
| 英文名称 | Phospho-STK4/3 (Thr183/Thr180) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化蛋白激酶MST重组兔单抗 |
| 别 名 | KRS1; KRS2; MST1; MST2; STK3; STK4; YSK3. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 2D13 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56 kDa |
| 检测分子量 | 60 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human STK4 around the phosphorylation site of T183: KRN-pT-VIG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. SWISS: Q13043 Gene ID: 6789 蛋白激酶MST(Mammalian Sterile20-like Kinase) 是各种组织均有表达的丝氨酸/苏氨酸（Serine/Threonine）蛋白激酶。属于人丝氨酸/苏氨酸激酶的哺乳动物STE20样激酶(MST)，MST1和MST2在caspase蛋白酶激活的细胞凋亡的上游和下游都起作用。 细胞凋亡中MST1被caspase蛋白酶切割和激活，能诱导细胞凋亡形态上的改变如染色质凝聚。哺乳动物STE20样激酶2 (Mst2) 与Mst1蛋白激酶很相似,有近90%的同源性。 |
| 产品图片 |
Ramos (H) cells were treated with or without Staurosporine (1μM) for 1h, Hela (H) cells were treated with or without Calyculin A (50nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-STK4/3 monoclonal antibody, unconjugated (bsm-62840R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
