| 产品编号 | bsm-62844R |
| 英文名称 | ITCH/AIP4 Recombinant Rabbit mAb |
| 中文名称 | 激活素受体相互作用蛋白4重组兔单抗 |
| 别 名 | ADMFD; AIF4; AIP4; NAPP1; 6720481N21Rik; 8030492O04Rik; A130065M08; C230047C07Rik; ITCH_HUMAN; ITCH; Atrophin-1-interacting protein 4 (AIP4); HECT-type E3 ubiquitin transferase Itchy homolog; NFE2-associated polypeptide 1 (NAPP1); 2.3.2.26; ITCH_MOUSE; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 103 kDa |
| 检测分子量 | 103 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ITCH: 800-850 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SWISS: Q96J02 Gene ID: 83737 |
| 产品图片 |
4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (ITCH/AIP4) monoclonal Antibody, unconjugated (bsm-62844R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ITCH/AIP4 antibody (bs-62844R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
