| 产品编号 | bsm-62853R |
| 英文名称 | phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化eIF4E结合蛋白1/2/3重组兔单抗 |
| 别 名 | eIF4EBP1 & eIF4EBP2 & eIF4EBP3 (p-T46/T46/T32); p-eIF4EBP1 & eIF4EBP2 & eIF4EBP3; phospho-eIF4EBP1 & eIF4EBP2 & eIF4EBP3; 4E-BP1; 4EBP1; BP-1; PHAS-I; 4EBP1_HUMAN; EIF4EBP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1 (PHAS-I); 4EBP1_MOUSE; 4EBP1_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 3A4 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11,13 kDa |
| 检测分子量 | 13-19 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human 4E BP1 around the phosphorylation site of T46: FST-pT-PGG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
eIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. SWISS: Q13541 Gene ID: 1978 4E-BP1是一种分子量为10-12kDa 的酸性蛋白，与eIF4G 竞争结合。4E-BPs 对 eIF4E 的结合是可逆的，并依赖于 4E-BP 的磷酸化状态。非磷酸化的4E-BP1 能与 eiF4E 牢固结合，而磷酸化的4E-BP1则不能。 而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通过对35、45、69位的苏氨酸残基或64位的丝氨酸残基进行磷酸化而使4E-BP1丧失对eIF4E结合能力的激酶。然而，并非所有的磷酸化事件都能 |
| 产品图片 |
Western blot analysis of 293 treated with FBS+293. Using Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) (bsm-62853R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
