| 产品编号 | bsm-62860R |
| 英文名称 | phospho-PKA C alpha/beta/gamma (T197) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化蛋白激酶A α/β/γ重组兔单抗 |
| 别 名 | PKA C alpha & beta & gamma (p-T197); p-PKA C alpha & beta & gamma; phospho-PKA C alpha & beta & gamma; BDPLT19; KAPG; PKACg; KAPCG_HUMAN; PRKACG; PKA C-gamma; 2.7.11.11; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 5B5 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40,41 kDa |
| 检测分子量 | 40 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PKA C alpha around the phosphorylation site of T197: RTW-pT-LCG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. SWISS: P22612 Gene ID: 5568 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C alpha/beta/gamma (T197) monoclonal antibody, unconjugated (bsm-62860R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Western blot analysis of HeLa cells, using ADCY1 antibody. Using Phospho-PKA C alpha/beta/gamma (T197) (bsm-62860R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha/beta/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
