| 产品编号 | bsm-62863R |
| 英文名称 | phospho-MAP2 (Ser136) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化微管相关蛋白2重组兔单抗 |
| 别 名 | MAP2 (p-S136); p-MAP2; phospho-MAP2; MAP-2; MAP2A; MAP2B; MAP2C; G1-397-34; Mtap-2; Mtap2; repro4; MAP2R; MTAP2_HUMAN; MAP2; MTAP2_MOUSE; MTAP2_RAT; |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 19F17 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 200 kDa |
| 检测分子量 | 70,280 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MAP2 around the phosphorylation site of S136: NLPP-pS-PPP(全长1827aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. SWISS: P11137 Gene ID: 4133 微管相关蛋白 2（MAP-2)是一种磷蛋白质，在正常脑组织中，主要存在于神经元的胞体、树突和树突棘，是脑内最丰富的蛋白之一。MAP-2在其调节微管的聚合作用和微管的稳定性以及对神经元轴突和树突的发生、延长、稳定和突触可塑性调节具有重要作用。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MAP2 (Ser136) Monoclonal Antibody, Unconjugated (bsm-62863R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
