| 产品编号 | bsm-62903R |
| 英文名称 | MAPKAP Kinase 2 Recombinant Rabbit mAb |
| 中文名称 | 丝裂原活化蛋白激酶活化的蛋白激酶2重组兔单抗 |
| 别 名 | MAPKAP K2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK2; Mitogen activated protein kinase activated protein kinase 2; MK 2. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 15C5 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | 46 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MK2: 350-400/400 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. SWISS: P49137 Gene ID: 9261 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with MAPKAP Kinase 2 monoclonal antibody, unconjugated (bsm-62903R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
