| 产品编号 | bsm-62926R |
| 英文名称 | OTX1/2 Recombinant Rabbit mAb |
| 中文名称 | 转录因子OTX1/2重组兔单抗 |
| 别 名 | CPHD6; MCOPS5; A730044F23Rik; jv; id:ibd2915; otx2; zOtx2; zgc:136535; zotx-2; OTX1_HUMAN; OTX1; Orthodenticle homolog 1; OTX2_HUMAN; Orthodenticle homolog 2; OTX1_MOUSE; Otx-1; OTX2_MOUSE; Otx-2; OTX2_DANRE; orthodenticle homeobox 1; orthodenticle (Drosophila) homolog 1; orthodenticle homolog 1 (Drosophila); Homeobox protein OTX1 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 5A6 |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37kDa |
| 检测分子量 | 37 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human OTX1: 20-70 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. SWISS: P32243 Gene ID: 5015 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with OTX1/2 monoclonal antibody, unconjugated (bsm-62926R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with OTX1/2 Monoclonal Antibody, Unconjugated (bsm-62926R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
