| 产品编号 | bsm-62977R |
| 英文名称 | SKP2 Recombinant Rabbit mAb |
| 中文名称 | 细胞S期激酶相关蛋白2重组兔单抗 |
| 别 名 | FBL1; FLB1; FBXL1; MGC1366; SKP2. |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | |
| 产品应用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | 48 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SKP2: 11-75 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. SWISS: Q13309 Gene ID: 6502 Skp2异常表达可加速细胞周期转化，该蛋白与肿瘤分化程度有关，Skp2参与细胞转化和肿瘤的形成，目前主要用于消化系统肿瘤方面的研究。 |
| 产品图片 |
Western blot analysis of (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate. Using SKP2 (bsm-62977R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
