| 产品编号 | bs-1208R |
| 英文名称 | ADAMTS1 Rabbit pAb |
| 中文名称 | 整合素样金属蛋白酶与凝血酶1型抗体 |
| 别 名 | A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin like and metalloprotease with thrombospondin type 1 motif 1; A disintegrin-like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 1; ADAM metallopeptidase |
| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 细胞类型标志物 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 106 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse ADAMTS1: 851-967/967 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ADAMTS1 is a metalloproteinase of the ADAM (A Disintegrin And Metalloproteinase) family containing disintegrin-like domains. ADAMTS1, also known as METH1, was first described as a protein elevated in invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor progression, since the protein was preferentially expressed in more invasive tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and organ morphology and function. Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. Subcellular Location: Secreted > extracellular space > extracellular matrix. Post-translational modifications: The precursor is cleaved by a furin endopeptidase. Similarity: Contains 1 disintegrin domain. Contains 1 peptidase M12B domain. Contains 3 TSP type-1 domains. SWISS: P97857 Gene ID: 11504 Database links: Entrez Gene: 9510 Human Entrez Gene: 11504 Mouse Omim: 605174 Human SwissProt: Q9UHI8 Human SwissProt: P97857 Mouse Unigene: 643357 Human Unigene: 8230 Human Unigene: 1421 Mouse Unigene: 7897 Rat |
| 产品图片 |
Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human 293T cell Lysates
Lane 3: Human U87-MG cell Lysates
Lane 4: Human U251 cell Lysates
Primary: Anti-ADAMTS1 (bs-1208R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106kDa
Observed band size: 80kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS1 Polyclonal Antibody, Unconjugated(bs-1208R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
