| 产品编号 | bs-1087R |
| 英文名称 | Angiopoietin 4 Rabbit pAb |
| 中文名称 | 血管生成素3/血管生成素4抗体 |
| 别 名 | Angiopoietin 4; AGP4; ANG3; ANG-4; ANG 4; ANGPT4; AGP4; ANGP4_HUMAN.ANG 3; ANG-3; ANG3; Angioarrestin; Angiopoietin 3; Angiopoietin like 1; Angiopoietin like protein 1; Angiopoietin related protein 1; Angiopoietin Y1; Angiopoietin-3; Angiopoietin-like protein 1; Angiopoietin-related protein 1; ANGL1_HUMAN; ANGPT3; ANGPTL1; AngY; ARP1; dJ595C2.2; KIAA0351; UNQ162. |
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Specific References (1) | bs-1087R has been referenced in 1 publications.
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[IF=1.922] Wang et al. Angiopoietin-like protein 4 improves glucose tolerance and insulin resistance but induces liver steatosis in high-fat-diet mice. (2016) Mol.Med.Rep. 14:3293-300 WB ; Mice. 222
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| 研究领域 | 肿瘤 心血管 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ANGPT4: 410-503/503 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Angiopoietin 4 is a glycosylated protein with a fibrinogen C terminal domain. It is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. Angiopoietin 4 is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. It may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. Alternatively spliced transcript variants encoding different isoforms have been described Function: Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis. Subunit: Homodimer; disulfide-linked. Interacts with TEK/TIE2. Subcellular Location: Secreted (Probable). Tissue Specificity: Highly expressed in the lung with much lower levels found in other tissues. Similarity: Contains 1 fibrinogen C-terminal domain. SWISS: Q9Y264 Gene ID: 51378 Database links: Entrez Gene: 51378 Human Omim: 603705 Human SwissProt: Q9Y264 Human Unigene: 278973 Human 血管生成素4-ANGPTL4与小血管生成、肿瘤的转移有很重要的关系。 |
| 产品图片 |
Tissue/cell: mouse tumor tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ANGPT3/ANG3/ANGPT4/ANG4 Polyclonal Antibody, Unconjugated(bs-1087R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
