| 产品编号 | bs-1145R |
| 英文名称 | ARF6 Rabbit pAb |
| 中文名称 | ADP核糖基化因子6抗体 |
| 别 名 | ARF6_CHICK; ARF6; 3.6.5.2; CPS1; ARF6_HUMAN; ARF6_MOUSE; ARF6_PIG; ARF6_RAT; ARF GTPase 6; ADP ribosylation factor 6 |
| 研究领域 | 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Chicken, Pig, Horse, Rabbit, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 20kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ARF6: 101-175/175 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. Function: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Subunit: Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Subcellular Location: Golgi apparatus. Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Cell membrane. Cell projection, filopodium membrane; Lipid-anchor. Note=Recruited to the cell membrane in association with CYTH2 and ARL4C. Similarity: Belongs to the small GTPase superfamily. Arf family. SWISS: P62330 Gene ID: 382 Database links: Entrez Gene: 382 Human Entrez Gene: 11845 Mouse Omim: 600464 Human SwissProt: P26990 Chicken SwissProt: P62330 Human SwissProt: P62331 Mouse Unigene: 525330 Human Unigene: 719973 Human Unigene: 27308 Mouse Unigene: 6993 Rat ARF6主要用于：肿瘤方面的研究，在很多肿瘤组织中都有一定的表达及分布。 技术背景：rRNA 加工、内质网高尔基膜泡介导运输、细胞运动性、细胞附着、小GTP 酶介导信号转导、蛋白运输、膜泡介导运输、正调控肌动蛋白丝聚合、皮层肌动蛋白细胞骨架组织和生物发生、褶边组织和生物发生、调控Rac 蛋白信号转导、负调控受体介导胞吞. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
