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RabbitAnti-CD34 Rabbit pAb  antibody (bs-0646R)
~~~促销，代码KXJ230206~~~
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说明书： 50ul  100ul  200ul
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产品编号 bs-0646R
英文名称 CD34 Rabbit pAb
中文名称 CD34抗体
别    名 CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.  
Specific References  (51)     |     bs-0646R has been referenced in 51 publications.
111 [IF=11.322] Zhenyin Chen. et al. Gelatin/sodium alginate composite hydrogel with dynamic matrix stiffening ability for bone regeneration. COMPOS PART B-ENG. 2022 Aug;243:110162  IF ;  Rat.  222
111 [IF=10.652] Pei Liu. et al. Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration. Chem Eng J. 2021 Aug;418:129531  IHC ;  Rabbit.  222
111 [IF=10.652] Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084  IF ;  Rabbit.  222
111 [IF=9.776] Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214  FC ;  Human.  222
111 [IF=8.786] Zhaoqi Zhang. et al. Central gene transcriptional regulatory networks shaping monocyte development in bone marrow.. FRONT IMMUNOL. 2022 Oct;13:1011279-1011279  FC ;  Mouse.  222
111 [IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  IF ;  Mouse.  222
111 [IF=7.129] Huifeng Yue. et al. Maternal NO2 exposure disturbs the long noncoding RNA expression profile in the lungs of offspring in time-series patterns. ECOTOX ENVIRON SAFE. 2022 Nov;246:114140  IHC ;  Mouse.  222
111 [IF=6.6] Hsueh-Chun Wang. et al. Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds. Cells-Basel. 2021 Dec;10(12):3536  FC ;  Human.  222
111 [IF=5.579] Chen et al. Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis. (2010) Mol.Cancer.The. 9:2164-74  IHC-F ;  Mouse.  222
111 [IF=5.546] Huang Z et al. The impact of acute stress disorder on gallbladder interstitial cells of Cajal. J Cell Physiol . 2020 Apr 24.  WB，IF ;  rabbit.  222
111 [IF=5.395] Zhenyin Chen. et al. Zonally Stratified Decalcified Bone Scaffold with Different Stiffness Modified by Fibrinogen for Osteochondral Regeneration of Knee Joint Defect. ACS BIOMATER-SCI ENG. 2022;XXXX(XXX):XXX-XXX  IF ;  Rabbit.  222
111 [IF=5.135] Ci Li. et al. Sustained release of exosomes loaded into polydopamine-modified chitin conduits promotes peripheral nerve regeneration in rats. Neural Regen Res. 2022 Feb;17(9):2050  FC ;  Rat.  222
111 [IF=4.982] Yuan, Liwei. et al. Bone marrow mesenchymal stem cells combined with estrogen synergistically promote endometrial regeneration and reverse EMT via Wnt/β-catenin signaling pathway. REPROD BIOL ENDOCRIN. 2022 Dec;20(1):1-13  FC ;  Rabbit.  222
111 [IF=4.963] Jin J et al. Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte.Stem Cell Res Ther. 2019 Mar 15;10(1):95.  ICF ;  Human.  222
111 [IF=4.963] Chen X et al. Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium.Stem Cell Res Ther. 2019 Mar 21;10(1):107.  IHC-P ;  Rat.  222
111 [IF=4.648] Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313  FCM ;  Rat.  222
111 [IF=4.627] Zhai L et al. Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes. Stem Cell Res Ther. 2020 Jan 3;11(1):10.  ICF ;  Mouse.  222
111 [IF=4.61] Yue H et al. Gestational exposure to PM2.5 impairs vascularization of the placenta. Sci Total Environ. 2019 May 15;665:153-161.  IHC-P&WB ;  Mouse.  222
111 [IF=4.47] Erin, Nuray, et al. "Differential characteristics of heart, liver, and brain metastatic subsets of murine breast carcinoma." Breast Cancer Research and Treatment (2013): 1-13.  Mouse.  222
111 [IF=4.197] Xuewen Qian. et al. Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a. Lab Invest. 2020 Aug;101(3):280-291  IHC ;  Human.  222
111 [IF=3.998] Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep . 2020 Jul 24;10(1):12448.  FCM ;  rat.  222
111 [IF=3.923] Sha Y et al. MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia‐induced cell apoptosis and accelerating angiogenesis.(2018) J Cell Physiol. 2018 Oct 14.  IF ;  Rabbit.  222
111 [IF=3.887] Yan LI. et al. The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1α and VEGF via the PI3K/AKT and ERK1/2 signaling pathways. CHIN J NAT MEDICINES. 2022 Sep;20:679  IF ;  Rabbit.  222
111 [IF=3.752] Lifen Wang. et al. Electroacupuncture attenuates ischemic injury after stroke and promotes angiogenesis via activation of EPO mediated Src and VEGF signaling pathways. PLOS ONE. 2022 Sep;17(9):e0274620  WB ;  Rat.  222
111 [IF=3.715] Nan Hou. et al. Tissue-engineered esophagus: recellular esophageal extracellular matrix based on perfusion-decellularized technique and mesenchymal stem cells. Biomed Mater. 2021 Aug;:  IHC ;  Rabbit.  222
111 [IF=3.485] Bai J et al. Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. Am J Physiol Cell Physiol . 2020 May 1;318(5):C1005-C1017.  FCM ;  rat.  222
111 [IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900  FCM ;  Human.  222
111 [IF=3.352] Qi Wang. et al. Telocytes in The Esophageal Wall of Chickens: A Tale of Subepithelial Telocytes. Poultry Sci. 2022 Mar;:101859  IHC ;  Chicken.  222
111 [IF=3.263] Mahmoud E. Youssef. et al. α7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996  IHC ;  Rat.  222
111 [IF=2.976] Guo H et al. Clinical associations between ASCT2 and p-mTOR in the pathogenesis and prognosis of epithelial ovarian cancer. Oncol Rep. 2018 Dec;40(6):3725-3733.  IHC-P ;  Human.  222
研究领域 肿瘤  心血管  免疫学  发育生物学  神经生物学  干细胞  细胞表面分子  糖蛋白  细胞类型标志物  血管内皮细胞  内皮细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:200-800 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 39 kDa
检测分子量
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD34: 301-385/385 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Function:
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Post-translational modifications:
Highly glycosylated.
Phosphorylated on serine residues by PKC.

Similarity:
Belongs to the CD34 family.

SWISS:
P28906

Gene ID:
947

Database links:

Entrez Gene: 947 Human

Omim: 142230 Human

SwissProt: P28906 Human

Unigene: 374990 Human



造血干细胞标志物
内皮标志物
肿瘤生物标志物
细胞表面的唾液粘蛋白。
间充质干细胞（mesenchymal stem cells,MSC）也是干细胞家族的重要成员，来源于发育早期的中胚层和外胚层。MSC最初在骨髓中发现，因其具有多向分化潜能、造血支持和促进干细胞植入、免疫调控和自我复制等特点。如间充质干细胞在体内或体外特定的诱导条件下，可分化为血管内皮、脂肪、骨、软骨、肌肉、肌腱、韧带、神经、肝、心肌、等多种组织细胞。
产品图片
25 ug total protein per lane of various lysates (see on figure) probed with CD34 polyclonal antibody, unconjugated (bs-0646R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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